Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

1. Prepare samples and controls., 2. Dilute 10X Injection Buffer 1:10 with 45 mL diH20., 3. Reconstitute SR-FLIVO with 50 µL DMSO., 4. Dilute SR-FLIVO 1:12 with 550 µL 1X Injection Buffer., 5. Inject 100 µL intravenously., 6. Let SR-FLIVO circulate 30-60 minutes., 7. View live tumor through a window chamber using a fluorescence microscope., 8. If not viewing directly, excise tissue., 9. If desired, label with additional stains, such as Hoechst 33342, or an antibody., 10. If desired, fix cells., 11. Analyze with a fluorescence microscope, flow cytometer, or a window chamber system. SR-FLIVO excites at 550-580 nm and emits at 590-600 nm.