Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay
A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a FAM dye and a FMK reactive entity.
Product Specifications
Assay Principle
1. Prepare samples and controls., 2. Dilute 10X Injection Buffer 1:10 with 45 mL diH20., 3. Reconstitute SR-FLIVO with 50 µL DMSO., 4. Dilute SR-FLIVO 1:12 with 550 µL 1X Injection Buffer., 5. Inject 100 µL intravenously., 6. Let SR-FLIVO circulate 30-60 minutes., 7. View live tumor through a window chamber using a fluorescence microscope., 8. If not viewing directly, excise tissue., 9. If desired, label with additional stains, such as Hoechst 33342, or an antibody., 10. If desired, fix cells., 11. Analyze with a fluorescence microscope, flow cytometer, or a window chamber system. SR-FLIVO excites at 550-580 nm and emits at 590-600 nm.
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC041231-100 1x 100 µL
Eosinophil Peroxidase (AHE-1), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC402848-100 1x 100 µL
Fibronectin (Fn-3), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680009-500 1x 500 µL
MART-1 / Melan-A (M2-7C10), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400012-100 1x 100 µL
Tyrosinase (T311), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details