Assay Diluent Optimization Pack

1. To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate., 2. Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types., 3. Make test spikes into serum samples., 4. Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples., 5. In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.