General Serum Diluent

1. Dilute ELISA Wash Buffer, 10X by adding 1 part ELISA Wash Buffer to 9 parts deionized water and mix for 15 minutes. ELISA Wash Buffer, 10X is supplied concentrated, so crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil., 2. Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 µL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system., 3. Aspirate or dump out the buffer and repeat for a total of 2-4 washes., 4. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid., If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.