General Serum Diluent
For diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid, and cell culture media, for use in sandwich ELISA. It can also be used for diluting many serum samples, including mammalian and chicken, in antigen-down ELISA.
Product Specifications
Assay Principle
1. Prepare standards and samples in General Serum Diluent., 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding., 3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 μL sample to 990 μL sample diluent for a total of 1,000 μL., 4. Highly concentrated samples may need to be diluted 1:1,000 or more., 5. Once diluted, run the assay according to the specific ELISA protocol.
Concentration
5X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
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