General Block ELISA Blocking Buffer
Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.
Product Specifications
Target Antigen
Necrotic and apoptotic cells
Assay Principle
If fluorescence-stained cells cannot be evaluated immediately after labeling with the fluorescence probes, add Fixative to the cells at a ratio of 1:10. For example, add 100 µL Fixative to 900 µL cells and incubate for at least 15 minutes at RT in the dark. Never add Fixative until the staining and final wash steps have been completed. Fixed cells may be stored on ice or at 4°C up to 24 hours.
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
CAS Number
7732-18-5
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