U-373 MG

Tumorigenecity: Yes, in nude mice; Grade III astrocytomas are formedIsoenzyme: PGM3, 1;PGM1, 1;ES-D, 1;G6PD, B;AK-1, 1;GLO-1, 1Histopathology: glioblastoma; AstrocytomaSubculture: Remove medium, add fresh 0.25 % Trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.Split ratio: Media change: Reverse transcritase: Production.