P3/NSI/1-Ag4-1; NS-1

This is a non-secreting clone of P3X63Ag8; Kappa chains are synthesized but not secreted; The cells are resistant to 0.1MM 8-azaguanine and do not grow in HAT medium; The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity; Tested and found negative for ectromelia virus (mouse pox)Tumorigenecity: noIsoenzyme: kappa light chainHistopathology: myeloma; PlasmacytomaSubculture: Split ratio: Media change: Reverse transcritase: Productionimmunoglobulin(not secreted).