Malme-3M

Tumorigenecity: Yes, in nude mice; Forms pigmented malignant melanomaIsoenzyme: Me-2, 1;PGM3, 1;PGM1, 1;ES-D, 1;AK-1, 1;GLO-1, 1-2;G6PD, BHistopathology: malignant melanoma; From metastatic siteSubculture: Remove medium, rinse with fresh 0.25% Trypsin solution, remove trypsin and let the culture sit at room temperature (or 37°C) until the cells detach (about 10min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.Split ratio: 1: 2 to 1: 4Media change: Twice per weekReverse transcritase: Production.