BALB/3T3 clone A31

Never allow cultures to become completely confluent before subculture. The serum used is calf serum, Not FBS. The serum initially employed and found satisfactory was from the Colorado Serum Co.; Tested and found negative for ectromelia virus(mouse pox).Tumorigenecity: noIsoenzyme: Histopathology: Subculture: Split ratio: Media change: Reverse transcritase: negativeProduction.