Dibenzylfluorescein

Dibenzylfluorescein (DBF) is a fluorogenic probe (Fluoresecent dye) that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19) . Dibenzylfluorescein is typically used near its Km value of 0.87-1.9 μM (Ex=485 nm, Em=535 nm) . Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease[1][2][3][4].

Product Specifications

CAS Number

[97744-44-0]

Product Name Alternative

DBF

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Cytochrome P450; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Applications

Metabolism-sugar/lipid metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/dibenzylfluorescein.html

Purity

99.54

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3C(C5=CC=CC=C5C(OCC6=CC=CC=C6)=O)=C2C=C1

Molecular Formula

C34H24O5

Molecular Weight

512.55

Precautions

H302, H315, H319, H335

References & Citations

[1]Stresser DM., et al. Substrate-dependent modulation of CYP3A4 catalytic activity: Analysis of 27 test compounds with four fluorometric substrates. Drug Metabolism and Disposition 28 (12), 1440-1448 (2000) . |[2]Donato MT., et al. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab. Dispos. 32 (7), 699-706 (2004) . |[3]Salminen KA, et al. Simple, direct, and informative method for the assessment of CYP2C19 enzyme inactivation kinetics. Drug Metabolism and Disposition 39 (3), 412-418 (2011) . |[4]Moutinho D, et al. Altered human CYP3A4 activity caused by Antley-Bixler syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system. Drug Metabolism and Disposition 40 (4), 754-760 (2012) .