Phytohemagglutinin

Phytohemagglutinin (PHA-M), the major seed lectin of the common bean, Phaseolus vulgaris, is a T-cell activator. Phytohemagglutinin stimulates human mononuclear leukocytes, inducing the expression of ChAT mRNA and potentiating ACh synthesis. Phytohemagglutinin induces dose- and time-dependent toxicity in THP-1 monocytes/macrophages, alters cellular morphology, causes organelle dysfunction, and increases the expression of NF-κB, COX2, IL-1β[1][2][3][4].

Product Specifications

CAS Number

[9008-97-3]

Product Name Alternative

PHA-M

UNSPSC

12352005

Target

COX; Interleukin Related; NF-κB; Reactive Oxygen Species (ROS)

Type

Natural Products

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB

Applications

Cancer-programmed cell death

Field of Research

Cancer; Neurological Disease; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/phytohemagglutinin.html

Purity

80.00

Solubility

H2O : 10 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

[Phytohemagglutinin]

References & Citations

[1]Prajitha N, et al. Intracellular inflammatory signalling cascades in human monocytic cells on challenge with phytohemagglutinin and 2,4,6-trinitrophenol. Mol Cell Biochem. 2022 Feb;477 (2) :395-414. |[2]Movafagh A, et al. The Significance Application of Indigenous Phytohemagglutinin (PHA) Mitogen on Metaphase and Cell Culture Procedure. Iran J Pharm Res. 2011 Fall;10 (4) :895-903. |[3]Fujii T, et al. Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator. J Neuroimmunol. 1998 Feb;82 (1) :101-107. |[4]Li L, et al. Response Gene to Complement 32 is associated with poor patient survival and an inflamed tumor-immune microenvironment in clear cell renal cell carcinoma. Transl Oncol. 2025 Feb;52:102248.