Recombinant Human Rock-2 Protein (Sumo Tag)

Product Specifications

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Abbreviation

Rock-2

Synonyms

ROCK 2; ROCK2; ROK alpha; ROKalpha; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; Rock II; Rock2; Rock2m

UniProt

O75116

Accession Number

O75116

Expression System

E.coli

Tag

N-Sumo

Sequence

Thr1212-Ser1388

Field of Research

Cell Biology; Cardiovascular; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.