Biotinylated Human HLA-A*11:01&B2M&EGF R (KITDFGLAK) Complex Protein (Monomer, MALS verified)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . Mutations affecting EGFR expression or activity could result in cancer. The Human HLA-A*1101 & B2M & EGF R (KITDFGLAK) protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and KITDFGLAK peptide of the EGF R.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*11:01&B2M&EGF R (KITDFGRAK) Complex Protein is produced by co-expression of HLA and B2M loaded with EGF R peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & KITDFGLAK

Expression Region

Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & KITDFGLAK peptide

Host

HEK293

Target

HLA-A*1101 & B2M & EGF R (KITDFGLAK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*11:01&B2M&EGF R (KITDFGLAK) Complex Protein (Cat. No. HLR-H82Ec) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.05-3 ng/mL (QC tested).