Anti-Pan-QKI Antibody

Mouse Monoclonal Antibody specific to Pan-QKI

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Hqk, HqkI

Gene Name

QKI

NCBI Gene ID

UniProt

Q96PU8

Cellular Locus

Nucleus, Cytoplasm

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1-341 (full-length) of human QKI-5. This sequence is 100

Target Antigen

Protein quaking

Target

Pan-QKI

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity). {UniProtKB:Q9QYS9, PubMed:16641098, PubMed:23630077}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~36-38kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat QKI-5. It cross-reacts with QKI-6, QKI-7, and QKI-7b.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

QKI (also called Protein Quaking or HqkI) is an RNA binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability, and protein translation and is itself regulated by alternative splicing. QKI is expressed in the frontal cortex of brain; it is shown to be downregulated in the brains of schizophrenic patients.