FireTaq™ DNA Polymerase

Biovision's FireTaq™ DNA Polymerase™ is a strategically-engineered, next generation Taq Polymerase that has rapid expansion rates, robust performance and contains a proprietary antibody that blocks polymerase activity at low temperatures. FireTaq™ Hot start Polymerase allows for a convenient reaction set-up at room temperature without non-specific amplification and primer-dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields and sensitivity while shortening reaction times by upto 70%, compared to wild-type Taq DNA Polymerase. During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. FireTaq™ has 5'-3' polymerase and 5'-3' exonuclease activities, lacks 3'-5' exonuclease activity, and produces 3'-dA-tailed amplicons. PCR products made with FireTaq™ can be used with TA cloning vectors.