Anti-Hu TdT PE

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

His-TdT recombinant protein

Target Antigen

TdT

Clone

41A

Applications

FC-IC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 7;24 (3) :376-389.e8., URL: https://www.ncbi.nlm.nih.gov/pubmed/30661959

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase; an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.