Fluoresterol

Fluoresterol is a fluorescently labeled cholesterol analog that can be used as a fluorescent probe to study the behavior and metabolism of cholesterol in cells, including lipid uptake and esterification analysis, intracellular localization and targeting, and lipid metabolism in mammalian and bacterial cells. Fluoresterol has an excitation maximum at 472 nm and an emission maximum at 540 nm. Fluoresterol is expected to be useful in the study of metabolic diseases[1][2].

Product Specifications

CAS Number

[78949-95-8]

Product Name Alternative

22-NBD Cholesterol

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Reference compound

Related Pathways

Others

Applications

Metabolism-sugar/lipid metabolism

Field of Research

Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/fluoresterol.html

Purity

99.65

Solubility

DMSO : 4.17 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CNC3=CC=C([N+]([O-])=O)C4=NON=C34)([H])[C@@]5([H])[C@]([C@@]6(C(C[C@@H](O)CC6)=CC5)C)([H])CC1

Molecular Formula

C28H38N4O4

Molecular Weight

494.63

Precautions

H302, H315, H319, H335

References & Citations

[1]Faletrov Y V, et al. 22-NBD-cholesterol as a novel fluorescent substrate for cholesterol-converting oxidoreductases[J]. The Journal of steroid biochemistry and molecular biology, 2013, 134: 59-66.|[2]Lajdova I, et al. Spectrally and time-resolved fluorescence imaging of 22-NBD-cholesterol in human peripheral blood mononuclear cells in chronic kidney disease patients[J]. Molecules, 2021, 26 (22) : 6800.