Recombinant rat Receptor-interacting serine/threonine-protein kinase 3 (Ripk3)
Product Specifications
Product Name Alternative
Homocysteine respondent protein HCYP2 (RIP-like protein kinase 3) (Receptor-interacting protein 3) (RIP-3) (Rip3)
Abbreviation
Recombinant Rat Ripk3 protein
Gene Name
Ripk3
UniProt
Q9Z2P5
Expression Region
1-478aa
Organism
Rattus norvegicus (Rat)
Target Sequence
MSSVKLWLNGASSISLVGSEELENLGFVGKGGFGAVFRARHTAWNLDVAVKIVNSKKISREVKAMVNLRHENVLLLLGVTENLEWDYVYGPALVTGFMENGSLSGLLQPSCPRPWPLLCRLLEEVVLGMCYLHSLNPSLLHRDLKPSNVLLDPELHAKLADFGLSTFQGGSQSGSGSGSRDSGGTLAYLAPELLDNDGKASKASDVYSFGVLVWTVLAGREAEVVDKTSLIRGAVCNRQRRPPLTELPPDSPETPGLEGLKELMTHCWSSEPKDRPSFQDCESKTNNVYILVQDKVDAAVSKVKHYLSQYRSSDTKLSARESSQKGTEVDCPRETIVYEMLDRLHLEEPSGSVPERLTSLTERRGKEASFGHATPAGTSSDTLAGTPQIPHTLPSRGTTPRPAFTETPGPDPQRNQGDGRNSNPWYTWNAPNPMTGLQSIVLNNCSEVQIGQHNCMSVQPRTAFPKKEPAQFGRGRGW
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
Baculovirus
Field of Research
Cancer
Relevance
Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production .
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
56.1 kDa
References & Citations
"A homocysteine-respondent gene cloned from WKY VSMCs by differential display." Chen K.H., Tang J. Submitted (MAY-2001)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Frequently Asked Questions
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