Recombinant Escherichia coli Microcin H47 (mchB)

Product Specifications

Product Name Alternative

(MccH47)

Abbreviation

Recombinant E.coli mchB protein

Gene Name

MchB

UniProt

P62530

Expression Region

16-75aa

Organism

Escherichia coli

Target Sequence

GGAPATSANAAGAAAIVGALAGIPGGPLGVVVGAVSAGLTTAIGSTVGSGSASSSAGGGS

Tag

N-terminal 10xHis-tagged

Type

CF Transmembrane Protein & In Stock Protein

Source

In vitro E.coli expression system

Field of Research

Cancer

Relevance

Bactericidal antibiotic. Active on bacteria phylogenetically related to the producing strain.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

10.9 kDa

References & Citations

"The structure, function, and origin of the microcin H47 ATP-binding cassette exporter indicate its relatedness to that of colicin V." Azpiroz M.F., Rodriguez E., Lavina M. Antimicrob. Agents Chemother. 45:969-972 (2001)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.