Phospho-Nucleolin (T76) Recombinant Rabbit Monoclonal Antibody [JE64-03]
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
C23 antibody FLJ45706 antibody MS1116 antibody NCL antibody Nucl antibody NUCL_HUMAN antibody Nucleolin antibody Protein C23 antibody
Abbreviation
NCL
Swiss Prot
P19338 Human
Cellular Locus
Nucleus, nucleolus;Cytoplasm
Host
Rabbit
Species Reactivity
Human
Immunogen
Recombinant protein within
Isotype
IgG
Clone
JE64-03
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
K-562 cell lysate, K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, human tonsil tissue, human kidney cancer tissue.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IHC-P: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 77 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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