Anti-Activating Transcription Factor 4 (ATF4) Antibody

Mouse Monoclonal Antibody specific to Activating Transcription Factor 4 (ATF4)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, TaxREB67

Gene Name

ATF4

NCBI Gene ID

UniProt

P18848

Cellular Locus

Nucleus, Nucleus speckle, Cytoplasm, Cell membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Host

Mouse

Reactivity

Human, Rat

Immunogen

Fusion protein corresponding to aa 25-327 of human ATF4. This sequence is 86

Target Antigen

Cyclic AMP-dependent transcription factor ATF-4

Target

Activating Transcription Factor 4 (ATF4)

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR) (PubMed:17684156, PubMed:16682973, PubMed:31444471, PubMed:32132707). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer (By similarity). Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress (PubMed:32132707). During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell rUniProtKB:Q06507, PubMed:11960987, PubMed:15109498, PubMed:15775988, PubMed:16682973, PubMed:17684156, PubMed:18940792, PubMed:26086088, PubMed:31444471, PubMed:32132706, PubMed:32132707, PubMed:33384352}.; (Microbial infection) Binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. {PubMed:1847461}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~60kDa is detected. Immunofluorescence: use at 10ug/mL.Detection of ATF4 in rat brain lysate with #56583. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and rat ATF4.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

ATF4 belongs to the large ATF/CREB family of transcription factors. These proteins bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers that regulate gene transcription. Two alternative transcripts encoding the same protein have been described. ATF4 transcription factor is also known to play role in osteoblast differentiation. Terminal osteoblast differentiation, is significantly inhibited by the inactivation of JNK; JNK inactivation downregulates expression of ATF-4.