Anti-Notch1 Antibody

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). {PubMed:15965470, PubMed:18299578, PubMed:23160044, PubMed:23615612}.

Immunoblotting: use at 1ug/mL. Bands of >270kDa, ~120kDa, and smaller fragments (due to proteolysis) are detected . <br><br>Positive control: Rat brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Members of the Notch trans-membrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. The Notch signaling network is an intercellular signaling pathway that regulates interactions between physically adjacent cells. The Notch1 protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. There is evidence that activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia. Notch 1, activated before birth, induces radial glia differentiation,but postnatally induces the differentiation into astrocytes.