Sheep anti Human TAK1

C-TAK1 (Cdc25C associated protein kinase) is a ubiquitously expressed protein kinase found in Human tissues and cell lines and can phosphorylate Cdc25C on serine 216 in vitro. C-TAK1 is distinct from the DNA damage checkpoint kinase Chk1 and may regulate interactions between Cdc25C and 14-3-3 in vivo by phosphorylating Cdc25C at serine 216.

Antibody detects c-tak1 by Western blot analysis at 1 to 5 µg/mL. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Peng, C.Y., et al. C-TAK1 protein kinase phosphorylates Human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Cell Growth Differ. 1998, 9, 197-208 2. Ogg, S., et al. Purification of a serine kinase that associates with and phosphorylates Human Cdc25C on serine 216. J. Biol. Chem. 1994, 269, 30461-30469 3. Busby, E.C., et al. The radiosensitizing agent 7-hydroxystaurosporine (UCN-01) inhibits the DNA damage checkpoint kinase hChk1. Cancer Res. 2000, 60, 2108-2112