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Monkeypox virus (strain Zaire-96-I-16) A33R Protein, His Tag

Monkeypox virus (strain Zaire-96-I-16) A33R Protein, His Tag (A3R-M52H5) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Asp 142 (Accession # Q8V4U6) .

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 88.7 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8V4U6

Expression Region

Met 1 - Asp 142

Host

HEK293

Target

A33R

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (strain Zaire-96-I-16)

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (strain Zaire-96-I-16) A33R Protein, His Tag (Cat. No. A3R-M52H5) at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody with a linear range of 1-63 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

88.7 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/A3R-M52H5.pdf

Species

Monkeypox virus (strain Zaire-96-I-16)

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