USP5 Recombinant Rabbit Monoclonal Antibody [JE77-42]
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
USP5, ISOT
Swiss Prot
P45974 Human, P56399 Mouse,
Cellular Locus
Cytosol, lysosome, nucleus.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within Human USP5 aa 759-858 / 858.
Isotype
IgG
Clone
JE77-42
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50 ;IHC-P: 1:200 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 96 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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