ALFA Selector ST monoclonal camelid affinity resin

HeLa mock lysate was mixed with recombinant purified ALFA-tagged GFP substrate (right lanes) . The mixture was incubated with either ALFA SelectorPE (left panel) or ALFA SelectorST (right panel) . After washing with PBS, bound proteins were eluted either with 200µM ALFA-peptide, glycin pH2.2 or SDS sample buffer (SDS) . Peptide elution under native conditions is efficient when using ALFA SelectorPE. The target protein can be recovered from both ALFA Selector resins by elution with glycin or SDS sample buffer.