Anti-Rat IgG2a Antibody, Alkaline Phosphatase conjugate
Goat Polyclonal Antibody specific to Rat IgG2a-Alk Phos
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-Rat IgG2a-Alk Phos Goat Polyclonal Antibody
Host
Goat
Reactivity
Rat
Immunogen
Rat IgG2a
Target
Rat IgG2a
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Secondary Antibodies
Purification Method
Purified by affinity chromatography on rat IgG2a
Concentration
0.5 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
HEPES
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000-1:10,000.
<br><br>Immunohistochemistry: use at a dilution of 1:100-1:1,000
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:1,000
<br><br>ELISA: use at a dilution of 1:1,000-1:10,000.
<br><br>These are recommended dilutions.
<br><br>End users should determine optimal dilutions for their applications. Prepare working dilutions immediately before use.
Storage Conditions
This antibody is stable for at least 1 year at 2-8°C.
Specificity
Antibody is affinity-purified on rat IgG2a and conjugated to alkaline phosphatase. Molar enzyme:antibody ratio = 1:1. This antibody may cross-react with IgG2a of other species.
Formulation
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
Buffer pH
pH 7.1
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