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Anti-MEF2a (phospho-Thr312) Antibody

Rabbit Polyclonal Antibody specific to MEF2a (phospho-Thr312)

Product Specifications

Product Name Alternative

Anti-MEF2a (phospho-Thr312) Rabbit Polyclonal Antibody

Gene Name

MEF2A

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=MEF2A">MEF2A</a>

UniProt

Q02078

Cellular Locus

Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from human MEF2a and conjugated to KLH.

Target Antigen

Myocyte-specific enhancer factor 2A

Target

MEF2a (phospho-Thr312)

Clonality

Polyclonal

Type

Antibody

Applications

IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. {PubMed:11904443, PubMed:12691662, PubMed:15834131, PubMed:16371476, PubMed:16484498, PubMed:16563226, PubMed:21468593, PubMed:9858528}.

Additionnal Information

<br><br>Immunohistochemistry: use at dilution of 1:50- 1:100. <br><br>Immunofluorescence: use at dilution of 1:100- 1:200. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/43039.pdf

Specificity

This antibody detects endogenous human, mouse, and rat MEF2a only when phosphorylated at threonine 312.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression including the DNA binding regulatory protein myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many muscle-specific genes. The MEF2 genes are members of the MADS gene family that also includes several homeotic genes and other transcription factors all of which share a conserved DNA-binding domain.

CAS Number

9007-83-4

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