ALX1 Double Nickase Plasmid

<strong>ALX1 Double Nickase Plasmid</strong>_x000D_ <strong>Catalog number:</strong> B2015424_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 10 µg_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> 2-8 °C_x000D_ <strong>Keywords:</strong> ALX1 Double Nickase Plasmid (h)_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F. Highly efficient generation of isogenic pluripotent stem cell models using prime editing Elife. 2022 Sep 7;11:e79208._x000D_ 2: McCaffrey J, Sibert J, Zhang B, Zhang Y, Hu W, Riethman H, Xiao M. CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis Nucleic Acids Res. 2016 Jan 29;44(2):e11._x000D_ 3: Sundaresan R, Parameshwaran HP, Yogesha SD, Keilbarth MW, Rajan R. RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a Cell Rep. 2017 Dec 26;21(13):3728-3739._x000D_ 4: Lee KZ, Mechikoff MA, Parasa MK, Rankin TJ, Pandolfi P, Fitzgerald KS, Hillman ET, Solomon KV. Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies ACS Synth Biol. 2022 Jan 21;11(1):53-60._x000D_ 5: He RL, Wu J, Cheng ZH, Li HH, Liu JQ, Liu DF, Li WW. Biomolecular Insights into Extracellular Pollutant Reduction Pathways of Geobacter sulfurreducens Using a Base Editor System Environ Sci Technol. 2022 Sep 6;56(17):12247-12256._x000D_ 6: Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser J. Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein Hum Gene Ther Methods. 2018 Jun;29(3):135-145._x000D_ 7: Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks Methods Enzymol. 2014;546:251-71._x000D_ 8: Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Sci Rep. 2016 Mar 31;6:23549._x000D_ 9: Horzempa J, Shanks RM, Brown MJ, Russo BC, O'Dee DM, Nau GJ. Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis J Microbiol Methods. 2010 Jan;80(1):106-8._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/9447599">10: Sebestyén MG, Ludtke JJ, Bassik MC, Zhang G, Budker V, Lukhtanov EA, Hagstrom JE, Wolff JA. DNA vector chemistry: the covalent attachment of signal peptides to plasmid DNA Nat Biotechnol. 1998 Jan;16(1):80-5. </a>_x000D_ _x000D_ <strong>Products Related to ALX1 Double Nickase Plasmid can be found at</strong> <a href="https://moleculardepot.com/product-category/DNA/"> DNA</a>