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Rabbit anti Human Nucleolar Protein 4

Product Specifications

Background

Novel methylation target specific for cervical cancer. Could be a marker in the early detection, risk prediction and etiologic research of cervial cancer. While the biological role of NOL4 in cervical cancer has yet to be determined, it is possible that it could be a potential tumor suppressor gene. It also appears to have important roles in cell cycle and apoptosis as well as functioning in ribosomal transcription and subunit assembly.

CAS Number

9007-83-4

Synonyms

NOL4

UniProt

O94818 (Human)

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

WB, ELISA, IHC

Field of Research

Cellular Structures

Purification Method

Ammonium Sulfate Precipitation

Assay Principle

Antibody can be used for Western blotting (1-5 µg/mL) and immunohistochemistry on paraffin embedded tissues (10 µg/mL). Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Ueki, N., et al. 'NOLP: identification of a novel Human nucleolar protein and determination of sequence requirements for its nucleolar localization.' Biochem Biophys Res Commun 1998;252:97-102. 2. Wang, S.S., et al. 'Identification of novel methylation markers in cervical cancer using restriction landmark genomic scanning.' Cancer Res 2008;68:2489-2497. 3. Visintin R, et al. 'The nucleolus: the magician's hat for cell cycle tricks.' Curr Opin Cell Biol 2000;12:372–7. 4. Olson MO, et al. 'The nucleolus: an old factory with unexpected capabilities.' Trends Cell Biol 2000;10:189–96.

Shipping Conditions

Ambient Temperature, freeze upon arrival

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

Functional Analysis

WB

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