Anti-Fibronectin Antibody

Mouse Monoclonal Antibody specific to Human Fibronectin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]

Gene Name

FN1

NCBI Gene ID

UniProt

P02751

Cellular Locus

Secreted, extracellular space, extracellular matrix

Host

Mouse

Reactivity

Human, Bovine

Immunogen

Human plasma fibronectin

Target Antigen

Fibronectin

Target

Fibronectin

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, Inhibition, IHC

Field of Research

Cell adhesion

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.

Additionnal Information

ELISA: 1-10ug/ml with immobilized antigen.Western Blot: 1-10ug/ml, reducing or non-reducing conditions.Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells. Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles