BI

BI is a derivative of DFHBI (Cat. No. B2797) that binds Broccoli™ with higher affinity and deliver improved cellular fluorescence. In cells, BI has been found to stabilize the Broccoli™ structure and promote its folding. Additionally, Broccoli™/BI complexes is significantly more photostable due to impaired light-induced photoisomerization, and rapid unbinding of photoisomerized cis-BI. BI is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Broccoli™ RNA tag without disrupting biological functions. Importantly, the optimized fluorescence properties of BI enable live single-molecule imaging of Broccoli™-tagged mRNA transcripts in mammalian cells.