Alpha-1 Acid Glycoprotein 1 (Human) ELISA Kit

Alpha 1 acid glycoprotein (AGP1), also known as orosomucoid, is an acute phase protein produced in liver cells and found at high levels in human plasma. AGP1 levels can change in response to certain drugs, pregnancy, and some disease states such as HIV infection. It is a carrier for basic molecules, protease inhibitors, and steroids in the blood. AGP1 has been recognized as one of four possible circulating biomarkers for estimating the five-year risk of all-cause mortality. BioVision’s Alpha 1 Acid Glycoprotein 1 ELISA Kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The OD value is proportional to the concentration of Human AGP1. The concentration of Human AGP1 in the samples can be calculated by comparing the OD of the samples to the standard curve.