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Anti-IAA | Indole 3 acetic acid

Product Specifications

Background

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

CAS Number

9007-83-4

Host

Rabbit

Reactivity

Arabidoppsis thaliana, Drosera capensis, Euphorbia pulcherrima, Medicago sativa(nodules)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

BSA-conjugated synthetic indole 3 acetic acid

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunolocalization (IL)

Dilution

1 : 5000-1 : 10 000 (ELISA), 1 : 100-1 :600 (IL)

Purity

Total IgG

Format

Lyophilized in PBS, Phosphate 10 mmol/l; NaCl 150 mmol/l at pH 7.35

References & Citations

LaPorta et al. (2019). Metamaterial architecture from a self-shaping carnivorous plant. Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):18777-18782. doi: 10.1073/pnas.1904984116.Kućkoet al. (2019). Spatio-temporal IAA gradient is determined by interactions with ET and governs flower abscission. J Plant Physiol. 2019 Mar 2;236:51-60. doi: 10.1016/j.jplph.2019.02.014.Nishimuraand Koshiba (2019). Immunolocalization of IAA Using an Anti-IAA-C-Antibody Raised Against Carboxyl-Linked IAA. Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY.Luet al. (2015). OsPIN5b modulates rice plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015 Jul 25. doi: 10.1111/tpj.12939.Biancoand Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.

Storage Conditions

Store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

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