DFHO

Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.