SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.86/V483/Denmark) (MALS verified)
SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.86/V483/Denmark) (SPD-C5248) is expressed from human 293 cells (HEK293) . It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, N460K, S477N, T478K, N481K, E484K, F486P, Q498R, N501Y, Y505H) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.86/V483/Denmark) .
Product Specifications
Background
It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
QHD43416.1 (I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, N460K, S477N, T478K, N481K, E484K, F486P, Q498R, N501Y, Y505H)
Expression Region
Arg 319 - Lys 537
Host
HEK293
Target
Spike RBD
Conjugation
Unconjugated
Tag
C-10xHis
Source
SARS-CoV-2
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD Protein, His Tag (BA.2.86/V483/Denmark) (Cat. No. SPD-C5248) with a linear range of 1.953-31.25 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
26.8 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/SPD-C5248.pdf
Species
SARS-CoV-2
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