3-Methyluridine

3-Methyluridine (m3U; N3-Methyluridine) is a methylated nucleotide present in ribosomal RNA (rRNA), mainly targeting specific base sites of RNA molecules such as 23S rRNA. 3-Methyluridine can introduce a methyl group at the N3 position of uracil, affecting the secondary structure stability and base pairing ability of RNA, and regulating ribosome function. For example, it affects ribosomal subunit binding and tRNA interaction. 3-Methyluridine is often used as a key raw material for the synthesis of modified nucleotides, and is used to construct RNA oligonucleotides containing methylation modifications to study the effects of RNA methylation on gene expression and drug resistance[1][2][3].

Product Specifications

CAS Number

[2140-69-4]

Product Name Alternative

N3-Methyluridine

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Endogenous Metabolite

Type

Natural Products

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/3-Methyluridine.html

Purity

99.05

Solubility

DMSO : 160 mg/mL (ultrasonic)

Smiles

O[C@H]1[C@@H](O)[C@H](N(C(N2C)=O)C=CC2=O)O[C@@H]1CO

Molecular Formula

C10H14N2O6

Molecular Weight

258.23

Precautions

H302, H315, H319, H335

References & Citations

[1]Rohan T Ranasinghe, et al. Detecting RNA base methylations in single cells by in situ hybridization. Nat Commun|[2]Desaulniers JP, et al. Solution conformations of two naturally occurring RNA nucleosides: 3-methyluridine and 3-methylpseudouridine. Bioorg Med Chem. 2005 Dec 15;13 (24) :6777-81.|[3]Chui HM, et al. Synthesis of a 3-methyluridine phosphoramidite to investigate the role of methylation in a ribosomal RNA hairpin. Bioorg Med Chem. 2002 Feb;10 (2) :325-32.