Biotinylated Human MICA (201-309) Protein, His, Avitag™

Biotinylated Human MICA (201-309) Protein, His, Avitag (MIA-H82Q7) is expressed from human 293 cells (HEK293) . It contains AA Leu 201 - Thr 309 (Accession # Q29983-1) .

Product Specifications

Background

MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.

Specifications

This protein carries a polyhistidine tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q29983-1

Expression Region

Leu 201 - Thr 309

Host

HEK293

Target

MICA

Conjugation

Biotin-labeled

Tag

N-6xHis & C-Avi

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.