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SingleFlowEx CD64 Alexa Fluor® 700

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

10.1

Applications

FC (QC tested)

Concentration

0.080 mg/mL

Format

Alexa Fluor® 700

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Devaraj S, Du Clos TW, Jialal I: Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects. Arterioscler Thromb Vasc Biol. 2005 Jul;25 (7) :1359-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/15860734, *Devaraj S, Davis B, Simon SI, Jialal I: CRP promotes monocyte-endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions. Am J Physiol Heart Circ Physiol. 2006 Sep;291 (3) :H1170-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/16603696, *Jayaram Y, Buckle AM, Hogg N: The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma. Clin Exp Immunol. 1989 Mar;75 (3) :414-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/2495203, *Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 , *Hashimoto S, Yamada M, Motoyoshi K, Akagawa KS: Enhancement of macrophage colony-stimulating factor-induced growth and differentiation of human monocytes by interleukin-10. Blood. 1997 Jan 1;89 (1) :315-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/8978307, *Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA: Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood. 1998 Jan 1;91 (1) :266-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/9414294, *Brichard B, Varis I, Latinne D, Deneys V, de Bruyere M, Leveugle P, Cornu G: Intracellular cytokine profile of cord and adult blood monocytes. Bone Marrow Transplant. 2001 May;27 (10) :1081-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11438825, *Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec;13 (12) :1571-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/11717198, *Beekman JM, Bakema JE, van der Linden J, Tops B, Hinten M, van Vugt M, van de Winkel JG, Leusen JH: Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin. J Biol Chem. 2004 Aug 6;279 (32) :33875-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/15161926, *Roura-Mir C, Wang L, Cheng TY, Matsunaga I, Dascher CC, Peng SL, Fenton MJ, Kirschning C, Moody DB: Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR-2. J Immunol. 2005 Aug 1;175 (3) :1758-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/16034117, *Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N: The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur J Immunol. 1987 Oct;17 (10) :1453-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3500057, *Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743, *Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94 (1) :23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353, *Use of this product has not been published., URL:

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

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