Goat anti-GFP Polyclonal antibody

Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 405. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.

Product Name Alternative

Green fluorescent protein antibody.

UNSPSC Description

Green Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Green Fluorescent Protein.

Immunogen

Purified recombinant peptide produced in E. coli.

Target Antigen

Purified recombinant peptide produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-GFP, DyLight®405

Clonality

Polyclonal

Isotype

IgG

Conjugation

DyLight®405

Type

Primary

Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

Applications

WB, IF, IHC-F

Purification

Epitope affinity purified

Concentration

2.5 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-F:1:50-1:1,000

Form

Polyclonal antibody supplied as a 100 µl (2.5 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.