Goat anti-tdTomato Polyclonal antibody

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It is a ~54 kDa protein that is optimally excited at a 554 nm and has a maximum of emission at 581 nm. It´s brightness and emission wavelength, makes it ideal for live animal research.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Product Name Alternative

red fluorescent protein antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

200 µL

Host

Goat

Reactivity

Reacts against tdTomato, mCherry, RFP

Immunogen

Purified recombinant peptide produced in E. coli.

Target Antigen

Purified recombinant peptide produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-tdTomato

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Format

Preservatives-Free

Form

Polyclonal antibody supplied as a 200 µl (2 mg/mL) aliquot in NaHCO3 100 mM pH 9. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.

Buffer

NaHCO3 100 mM pH 9; no preservatives

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.