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Goat anti-TagRFP Polyclonal antibody

Goat polyclonal antibody to TagRFP (orange fluorescent protein). TagRFP is a basic (constitutively fluorescent) fluorescent protein, monomeric engineered derivate of fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It has very low acid sensitivity. TagRFP is a ~26 kDa protein that is optimally excited at a 555 nm and has a maximum of emission at 584 nm. This fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.

Product Specifications

CAS Number

9007-83-4

Specifications

In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.

Product Name Alternative

RFP antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Reacts with Red Fluorescent Protein

Immunogen

Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Target Antigen

Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-TagRFP

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MASSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHR

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

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