Goat anti-Sapphire Polyclonal antibody
Goat polyclonal antibody to Sapphire. Sapphire is a basic (constitutively fluorescent) long stokes shift fluorescent protein engineered from green fluorescent protein (GFP) isolated from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with low acid sensitivity. Sapphire is a ~27 kDa protein that is optimally excited at a 399 nm and has a maximum of emission at 511 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Product Specifications
CAS Number
9007-83-4
Specifications
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Product Name Alternative
green fluorescent protein antibody.
UNSPSC Description
Green Fluorescent Protein
Volume
100 µL
Host
Goat
Reactivity
Reacts against Green Fluorescent Protein
Immunogen
Purified recombinant fluorescent protein produced in E. coli.
Target Antigen
Purified recombinant fluorescent protein produced in E. coli.
Immunogen Type
Recombinant protein
Target
anti-Sapphire
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Type
Primary
Applications
WB, IF, IHC-P, IHC-F, IEM
Purification
Epitope affinity purified
Concentration
3 mg/mL
Dilution
WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Form
Buffer
PBS, 20% glycerol and 0.05% sodium azide
Storage Conditions
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