Goat anti-mRuby Polyclonal antibody

Goat polyclonal antibody to mRuby (Red fluorescent protein). mRuby is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.

Product Specifications

CAS Number

9007-83-4

Specifications

In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.

Product Name Alternative

far-Red antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Reacts against Red Fluorescent Protein

Immunogen

Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Target Antigen

Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-mRuby

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.