Goat anti-mApple Polyclonal antibody

Goat polyclonal antibody to mApple (Apple fluorescent protein). mApple is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Product Name Alternative

red fluorescent protein, Apple antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Reacts against Red Fluorescent Protein

Immunogen

Purified recombinant fluorescent protein produced in E. coli.

Target Antigen

Purified recombinant fluorescent protein produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-mApple

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.