Goat anti-pHluorin Polyclonal antibody

Goat polyclonal antibody to pHluorin (pHluorin fluorescent protein). pHluorin is a multistate long stokes shift of green fluorescent protein engineered from green fluorescent protein (GFP) isolated from Aequorea victoria. pHluorin is a ~27 kDa protein that is optimally excited at a 475/395 (acid/alkaline) nm and has a maximum of emission at 509 nm (acid/alkaline). It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).

Product Name Alternative

green fluorescent protein antibody.

UNSPSC Description

Green Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Reacts against Green Fluorescent Protein

Immunogen

Purified recombinant fluorescent protein produced in E. coli.

Target Antigen

Purified recombinant fluorescent protein produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-pHluorin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.