Goat anti-pHluorin Polyclonal antibody
Goat polyclonal antibody to pHluorin (pHluorin fluorescent protein). pHluorin is a multistate long stokes shift of green fluorescent protein engineered from green fluorescent protein (GFP) isolated from Aequorea victoria. pHluorin is a ~27 kDa protein that is optimally excited at a 475/395 (acid/alkaline) nm and has a maximum of emission at 509 nm (acid/alkaline). It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Product Specifications
Specifications
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Product Name Alternative
green fluorescent protein antibody.
UNSPSC Description
Green Fluorescent Protein
Volume
100 µL
Host
Goat
Reactivity
Reacts against Green Fluorescent Protein
Immunogen
Purified recombinant fluorescent protein produced in E. coli.
Target Antigen
Purified recombinant fluorescent protein produced in E. coli.
Immunogen Type
Recombinant protein
Target
anti-pHluorin
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Type
Primary
Applications
WB, IF, IHC-P, IHC-F, IEM
Purification
Epitope affinity purified
Concentration
3 mg/mL
Dilution
WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Form
Buffer
PBS, 20% glycerol and 0.05% sodium azide
Storage Conditions
CAS Number
9007-83-4
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