Goat anti-Spike RBD Domain (SARS-CoV-2) Polyclonal antibody

The spike or S glycoprotein is a transmembrane protein with a molecular weight of about 150 kDa found in the outer portion of the SARS-CoV-2 virus. This protein is composed of two subunits, S1 and S2 and plays a key role in the receptor recognition and cell membrane fusion process. The S1 subunit contains a receptor-binding domain that recognizes and binds to the host receptor angiotensin-converting enzyme 2, while the S2 subunit mediates viral cell membrane fusion.

Product Specifications

CAS Number

9007-83-4

Specifications

In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.

Product Name Alternative

S glycoprotein SARS Coronavirus-2, Spike RBD Domain SARS Coronavirus-2 antibody.

Volume

100 µL

Host

Goat

Antigen Species

SARS-CoV-2

Reactivity

Reacts with SARS-CoV-2 protein

Immunogen

Affinity purified recombinant fusion protein using the spike protein RBD domain (residues 343 to 400) and produced in E. coli.

Target Antigen

Affinity purified recombinant fusion protein using the spike protein RBD domain (residues 343 to 400) and produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-Spike RBD Domain (SARS-CoV-2)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADV

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.