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Goat anti-Nucleolin Polyclonal antibody

Goat polyclonal antibody to Nucleolin. NCL is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is found associated with pre-ribosomal particles and intranucleolar chromatin. May play a role in the process of transcriptional elongation and chromatin decondensation by binding to histone H1.

Product Specifications

Specifications

Detects endogenous levels of NCL by Western blot.

Product Name Alternative

B530004O11Rik, C23, D0Nds28, D1Nds28, Nucl antibody.

UNSPSC Description

Nucleolin

Volume

100 µL

Gene ID

ENSG00000115053

Accession Number

ENSG00000115053

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Nucleolin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

TLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

Applications

WB

Purification

Epitope affinity purified

Concentration

1 mg/mL

Dilution

WB:1:1,000-1:5,000

Form

Polyclonal antibody supplied as a 100 µl (1 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

CAS Number

9007-83-4

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