Goat anti-Vimentin Polyclonal antibody

Goat polyclonal antibody to Vimentin. VIM is a member of the intermediate filament family. The cytoskeleton is made by these intermediate filaments, along with actin microfilaments and microtubules. VIM is responsible for integrity of the cytoplasm, stabilization of the cytoskeletal interactions and maintaining cell shape. This protein controls the transport of LDL-derived cholesterol from a lysosome to the site of esterification and is also involved in the immune response. It functions as an organizer of a number of critical proteins involved in migration, attachment and cell signaling.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects endogenous levels of VIM by Western blot.

Product Name Alternative

CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM antibody.

UNSPSC Description

Vimentin

Volume

100 µL

Gene ID

ENSG00000026025

Accession Number

ENSG00000026025

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Recombinant peptide derived from within residues 390 aa to the C-terminus of human VIM produced in E. coli.

Target Antigen

Recombinant peptide derived from within residues 390 aa to the C-terminus of human VIM produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Vimentin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

ALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000; IF:1:100-1:500

Form

Polyclonal antibody supplied as a 100 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Galderisi S, Cicaloni V, Milella MS, et al. J Cell Physiol 2021 Jan. PMID: 33469937

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.